It was anticipated that such resources would greatly facilitate large-scale parallel analyses of gene expression studies aimed at localizing the site of expression of all BMAP transcripts in the brain. One of the aims in the first phase of this project was the discovery of most transcripts expressed in the mouse brain, and the development of a comprehensive nonredundant arrayed collection of BMAP cDNAs and expressed sequence tags (ESTs). The Brain Molecular Anatomy Project (BMAP) was initiated in 1998 by the National Institute of Mental Health (NIMH) and the National Institute of Neurological Disorders and Stroke (NINDS) as an interdisciplinary project to establish state-of-the-art technologies and informatics systems to decipher the molecular anatomy of the mammalian brain. Importantly, we identified transcripts, among the 1274 full-ORF sequences, that are exclusively or predominantly expressed in brain and eye tissues, many of which encode yet uncharacterized proteins. We show that NIH_BMAP clones comprise 68% of mouse MGC cDNAs ≥5 kb, and 54% of those ≥4 kb, as of March 15, 2004. Altogether, including the full-ORF MGC sequences derived from these libraries by the MGC sequencing team, NIH_BMAP full-ORF sequences correspond to ∼20% of all transcripts currently represented in mouse MGC. cDNA libraries were derived from size-fractionated cytoplasmic mRNA isolated from brain and eye tissues obtained at several embryonic stages and postnatal days. Here we report the analysis of the ORF sequence of 1274 cDNAs, obtained from 47 full-length-enriched cDNA libraries, constructed by using a novel approach, herein described. As part of the trans-National Institutes of Health (NIH) Mouse Brain Molecular Anatomy Project (BMAP), and in close coordination with the NIH Mammalian Gene Collection Program (MGC), we initiated a large-scale project to clone, identify, and sequence the complete open reading frame (ORF) of transcripts expressed in the developing mouse nervous system.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |